***招生专业***：欢迎报考药学、分析化学、生物化工、物理化学等专业硕/博士研究生

教育工作经历

2017.04-至今       学院，新葡萄3522登录网页版，研究员

2011.11-2017.03  美国西南医学中心（UT Southwestern Medical Center，TX USA），博士后

2009.07-2011.10  中科院福建物质结构研究所，博士后

2006.09-2009.07  中科院上海药物研究所，药物设计学/博士

2003.09-2006.07  福建农林大学，生物化学与分子生物学/硕士

1999.09-2003.07  福建农林大学，生物科学/学士

教学简介

 本科 《分析化学》

 研究生 《化学生物学前沿》

科研简介

本课题组近年来以X-射线晶体学和冷冻电镜技术为主要研究手段，结合生物化学、分子生物学、细胞生物学以及计算生物学等研究方法，探索了核酸水解酶在DNA损伤修复和细菌CRISPR免疫系统中的重要功能和工作机制。此外，我们还以这些蛋白质为靶标开展了小分子化合物的高通量筛选，获得了若干个具有抗肿瘤、抗病毒和抗菌活性的候选小分子药物。相关研究成果以第一作者或通讯作者发表在Nature，Nature Chemical Biology，Nucleic Acids Research, EMBO Journal等国际主流刊物上。

社会兼职 

科研项目

 国家自然科学基金委面上项目（2020.01-2023.12）

 福建省百人计划项目（2018年）

 福建高校领军人才项目（2018年）

 国家高层次青年人才启动项目（2017年）  

代表性论文

2023年

Liyang Du, Qinwei Zhu and Zhonghui Lin^*.Molecular mechanism of allosteric activatation of the CRISPR ribonuclease Csm6 by cyclic tetra-adenylate.EMBO Journal.2023 Dec 19 DOI:10.1038/s44318-023-00017-w

Xu Zhang, Xuening Chen, Lian Lu, Qianqian Fang, Chun Liu, Zhonghui Lin*.Identification of small-molecule inhibitors of human MUS81-EME1/2 by FRET-based high-throughput screening.Bioorg Med Chem.2023,90:117383.doi: 10.1016/j.bmc.2023.117383.

Xu Zhang#, Zixuan Zhou#, Lin Dai, Yulin Chao, Zheng Liu, Mingdong Huang, Qianhui Qu^*, Zhonghui Lin^*.Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa.Front. Plant Sci.2023,14:1139106. doi: 10.3389/fpls.2023.1139106.

Liyang Du, Danping Zhang, Zhipu Luo*, Zhonghui Lin*.Molecular basis of stepwise cyclic tetra-adenylate cleavage by the type III CRISPR ring nuclease Crn1/Sso2081. Nucleic Acids Research.2023,51(5):2485-2495.doi:10.1093/nar/gkad101.

方倩倩#，陈浩#，廖馨源，林忠辉*．限制性核酸内切酶Asc I 蛋白质的表达纯化、酶活测定及小角散射结构解析[J/OL]．中国生物化学与分子生物学报.2023-02-07.https://doi.org/10.13865/j.cnki.cjbmb.2022.12.1482.

2022年

Dai L, Lu L, Zhang X, Wu J, Li J, Lin Z*.Identification of small-molecule inhibitors of the DNA repair proteins RuvAB from Pseudomonas aeruginosa.Bioorg Med Chem. 2022,73:117022. doi: 10.1016/j.bmc.2022.117022.

He Y, Fu W, Du L, Yao H, Hua Z, Li J, Lin Z*. Discovery of a novel Aurora B inhibitor GSK650394 with potent anticancer and anti-aspergillus fumigatus dual efficacies in vitro.J Enzyme Inhib Med Chem. 2022,37:109-117. doi: 10.1080/14756366.2021.1975693.

Yang DC, Wen LF, Du L, Luo CM, Lu ZY, Liu JY*, Lin Z*. A Hypoxia-Activated Prodrug Conjugated with a BODIPY-Based Photothermal Agent for Imaging-Guided Chemo-Photothermal Combination Therapy.ACS Appl Mater Interfaces.2022,14:40546-40558. doi: 10.1021/acsami.2c09071.

Lu L, Peng Y, Yao H, Wang Y, Li J*, Yang Y*, Lin Z*. Punicalagin as an allosteric NSP13 helicase inhibitor potently suppresses SARS-CoV-2 replication in vitro.Antiviral Res. 2022,206:105389. doi: 10.1016/j.antiviral.2022.105389.

Yang DC, Yang XZ, Luo CM, Wen LF, Liu JY*, Lin Z*. A promising strategy for synergistic cancer therapy by integrating a photosensitizer into a hypoxia-activated prodrug.Eur J Med Chem. 2022,243:114749. doi: 10.1016/j.ejmech.2022.114749.

Fang Q, Hua Z, Lin Z*. A protocol to determine the activities of human MUS81-EME1&2 endonucleases.STAR Protoc. 2022 ,3:101528. doi: 10.1016/j.xpro.2022.101528.

Hua Z, Fang Q, Zhang D, Luo Z, Yuan C, Lin Z*. Crystal structure of the human MUS81-EME2 complex.Structure. 2022,30:743-752.e3. doi: 10.1016/j.str.2022.02.015.

2021年

Yang DC, Wang S, Weng XL, Zhang HX, Liu JY*, Lin Z*. Singlet Oxygen-Responsive Polymeric Nanomedicine for Light-Controlled Drug Release and Image-Guided Photodynamic-Chemo Combination Therapy.ACS Appl Mater Interfaces. 2021,13:33905-33914. doi: 10.1021/acsami.1c09044.

2020年

Hu Y, He Y, Lin Z*.Biochemical and structural characterization of the Holliday junction resolvase RuvC from Pseudomonas aeruginosa.Biochem Biophys Res Commun. 2020,525:265-271. doi: 10.1016/j.bbrc.2020.02.062.

2019年

Lin H, Zhang D, Zuo K, Yuan C, Li J*, Huang M*, Lin Z*.Structural basis of sequence-specific Holliday junction cleavage by MOC1.Nat Chem Biol. 2019,15:1241-1248. doi: 10.1038/s41589-019-0377-4. 

2018年

Lee HS, Lin Z, Chae S, Yoo YS, Kim BG, Lee Y, Johnson JL, Kim YS, Cantley LC, Lee CW, Yu H, Cho H.The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.Nat Commun. 2018,9:3848. doi: 10.1038/s41467-018-06377-w.

2017年以前

Zhonghui Lin, Xuelian Luo, Hongtao Yu*. Structural basis of cohesin cleavage by separase. Nature. 2016, 532: 131-134.

Zhonghui Lin#, Luying Jia#, Diana R. Tomchick, Xuelian Luo, Hongtao Yu*. Substrate-specific activation of the mitotic kinase Bub1 through intramolecular autophosphorylation and kinetochore targeting. Structure. 2014, 22:1-12.

Zhonghui Lin#, Jan K. Jensen#, Zebin Hong, Xiaoli Shi, Lihong Hu, Peter A. Andreasen, and Mingdong Huang*. Structural insight into inactivation of plasminogen activator inhibitor-1 by a small-molecule antagonist. Chemistry & Biology. 2013, 20:253-61.

Zhonghui Lin, Longguang Jiang, Cai Yuan, Jan K.Jensen, Xu Zhang, Zhipu Luo, Barbara C. Furie, Bruce Furie, Peter A. Andreasen, Mingdong Huang*. Structural basis for recognition of urokinase-type plasminogen activator by plasminogen activator inhibitor-1. J. Biol. Chem. 2011, 286: 7027-32. 

Zhonghui Lin, Yu Zhang, Yinan Zhang, Hong Shen, Lihong Hu, Hualiang Jiang, and Xu Shen*. Oleanolic acid derivative NPLC441 potently stimulates glucose transport in 3T3-L1 adipocytes via multi-target mechanism. Biochemical Pharmacology. 2008, 76:1251-62.

 

获奖情况

学院杰出青年教师励志奖（2021）

学院青年五四奖章（2019年）

福建省“向上向善育人工程基金”奖教金 (2019年)

美国西南医学中心药理系年度优秀博士后（2016年）

国际血栓与止血协会“第三世界科学家奖”（2011年）

其他